Coding

Part:BBa_K1777017:Design

Designed by: Xilin Jiang   Group: iGEM15_Fudan   (2015-09-17)

NOTED

Because our sequencing data suggest this part is not compatible, we resent parts and redocument this part as BBa_K1777005”
click here to BBa_K1777005


flexible linker with SV40 NLS


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Design Notes

The codon is optimized for mammalian, and we insert a NLS to promote locating in cell nucleus. Besides ,we lengthen this linker to provide better separation of two domains. At last we avoid tandem repeat sequence(like GGGSPKKKRKVGGGS), which will facilitate fusing this linker to your domains via overlap PCR.


Source

This part is improved from BBa_K1486003.

References